TRAININGS
We offer training on the following:
1. Flow cytometry and cell sorting theoretical class - COMPULSORY
2. BD Symphony A1 analyzer
3. BD FACS Melody cell sorter - Training on the sorter will only be available to those that have received training on the Symphony A1 analyzer. This instrument is used for sorting only.
4. Data analysis software training
Flow cytometry theoretical training
Sign up for a flow cytometry theoretical class
Theoretical classes will commence every 2 weeks at 10-12pm at 227 ERML building.
Download the presentation for general flow cytometry here
Download the presentation for cell sorting here
*Please note: You are required to take a quiz before and after the theory session to assess you knowledge before and after your class.
This Training consists of:
1. Basics of flow cytometry - Flow cytometry principle and how an analyzer works
2. Cell sorting - Cell sorting principle, types of sorts, sorting preview on the BD FACS Melody
Please complete the follow quiz before AND after your theory training
Which instrument/s will you be trained on?
Training on the Symphony A1 Analyzer
Follow the steps below to set up a training session on our analyzer:
1. Fill out the user registration form
https://biotech.illinois.edu/cmto/user registration form
2. Familiarize yourself with the instrument before the training session using the following links
https://biotech.illinois.edu/cmto/services-eqiupment/bd-facsymphony-a1-cell-analyzer
https://biotech.illinois.edu/sites/biotech.illinois.edu/files/uploads/Symphony%20User%20Guide.pdf
Training on the BD FACS Melody Cell Sorter
Please have a look at the configuration of the BD FACS Melody sorter using table below and ensure that you have fluorophore's that are excited by the lasers present in the system. Please let us know if you need help in finding your fluorophore
Please note the BD FACS Melody training will take place over a course of 4 different training sessions. This is to ensure that the user is fully trained before being left alone with the instrument.
Day 1 - A CMtO employee will explain how to use the instrument with a short demonstration
Day 2 - Run your samples with the help of an employee
Day 3 - You will run your own samples with the occasional help of an employee
Day 4 - You will independently run your sort , from instrument start up to instrument shut down with the supervision of an employee
You can have a look at the detailed training schedule here
Please note: You can only use the instrument independently and gain access to the calendar once an employee clears you safe to use the instrument. If you are not cleared, you will be required to repeat day 4 until you are cleared. These 4 days can be spaced out over two weeks according to a CMtO employee and your schedule. Each day will take between 1-3 hours depending on your samples (this would be clarified before your trainings are scheduled). Please compensate time for any troubleshooting as well.
Familiarize yourself with the instrument before the training session
Quick troubleshooting on the BD FACS Melody
Additional Training materials
Introduction to flow cytometry
Experimental design on DIVA software
Manufacturer: BD Biosciences
Equipment Model: Symphony A1/ FACS Melody
Location: Main Facility at 231 Edward R. Madigan Laboratory and 264 Roger Adams Lab
Contact: cmto-core@mx.uillinois.edu